Journal: NPJ Vaccines
Article Title: A system to rapidly develop a bunyavirus pseudotyped virus neutralisation assay for pandemic preparedness
doi: 10.1038/s41541-025-01278-8
Figure Lengend Snippet: 100 TCID 50 of RVFV (green) CCHFV (blue) DABV (orange) or OROV (black) VSV-based PV were incubated with serial dilutions of anti-RVFV mAb-144 ( A ), anti-CCHFV mAB-11E7 ( B ), anti-DABV S2A5 ( C ) or anti-OROV ascitic fluid ATCC VR1228AF ( D ), prior to addition to Huh7 cells. Percentage of neutralisation was calculated based on luciferase activity relative to PV only. The graphs show average values ± standard error of the mean of at least two experiments conducted in duplicate.
Article Snippet: 100 TCID 50 of RVFV (green) CCHFV (blue) DABV (orange) or OROV (black) VSV-based PV were incubated with serial dilutions of anti-RVFV mAb-144 ( A ), anti-CCHFV mAB-11E7 ( B ), anti-DABV S2A5 ( C ) or anti-OROV ascitic fluid ATCC VR1228AF ( D ), prior to addition to Huh7 cells.
Techniques: Incubation, Luciferase, Activity Assay